rabbit anti eif4g (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
rabbit anti eif4g
Rabbit Anti Eif4g, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 352 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eif4g/eIF4G+Antibody/pm41917183-228-30-72
Average 95 stars, based on 352 article reviews
Rabbit Anti Eif4g, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 352 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eif4g/eIF4G+Antibody/pm41917183-228-30-72
Average 95 stars, based on 352 article reviews
rabbit anti eif4g - by Bioz Stars,
2026-09
95/100 stars
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Blocking Assay:Article Title: Arginine synthesis pathway and ASS1 play a critical role in mRNA translation reprogramming and ICI resistance in cutaneous melanoma Article Snippet: The PLA protocol was followed according to the manufacturer’s instructions (Olink, Sigma-Aldrich). .. After blocking, the antibodies were used at the following concentrations: eIF4E (mouse, clone A-10, 1:200; SantaCruz Biotechnology, sc-271480) and Article Title: Methods and compositions for treating androgen receptor deficient, androgen receptor low, and castration-resistant prostate cancers Article Snippet: FFPE sections were deparaffinized, rehydrated, and subjected to antigen retrieval in a decloaking chamber at 95° C. for 30 min in Tris-based antigen unmasking solution, pH 9 (Vector). .. After 30 min blocking, primary antibody incubation was performed at 4° C. overnight: eIF4E (SCBT), Article Title: Methods and compositions for treating androgen receptor deficient, androgen receptor low, and castration-resistant prostate cancers Article Snippet: .. Permeabilized cells were washed using 1× wash buffer followed by 1 hour blocking in a humidity chamber at 37° C. Next, cells were incubated with primary antibodies: Incubation:Article Title: Arginine synthesis pathway and ASS1 play a critical role in mRNA translation reprogramming and ICI resistance in cutaneous melanoma Article Snippet: The PLA protocol was followed according to the manufacturer’s instructions (Olink, Sigma-Aldrich). .. After blocking, the antibodies were used at the following concentrations: eIF4E (mouse, clone A-10, 1:200; SantaCruz Biotechnology, sc-271480) and Article Title: Cytoskeletal protein KRT14 governs cisplatin resistance by modulating eIF4H-dependent ACOX2 translation and lipid metabolism in bladder cancer. Article Snippet: Equal amounts of protein (20–40 μg) were separated on 4–15% MiniPROTEAN TGX gels (Bio-Rad 4561086) and transferred to 0.45 μm PVDF membranes (Millipore IPVH00010). .. Membranes were blocked in TBST containing 5% non-fat dry milk or 5% BSA for 1 h at room temperature, then incubated overnight at 4 °C with primary antibodies: KRT14 (1:1,000; Abcam ab181595), ACOX2 (1:1,000; Proteintech 10143-1-AP), eIF4H (1:1,000; CST 3469), Article Title: Methods and compositions for treating androgen receptor deficient, androgen receptor low, and castration-resistant prostate cancers Article Snippet: Equal amounts of proteins were subjected to SDS-PAGE and transferred to PVDF membranes (Bio-Rad). .. Membranes were blocked with 5% non-fat milk in PBS-Tween and were subsequently incubated with the following antibodies at 4° C. overnight: AR (N-terminus, Abcam), AR (C-terminus, SCBT), AR (GeneTex), p-AKT (S473, CST), p-AKT (T308, CST), eIF4E (BD Biosciences), eIF4A (CST), Article Title: Methods and compositions for treating androgen receptor deficient, androgen receptor low, and castration-resistant prostate cancers Article Snippet: FFPE sections were deparaffinized, rehydrated, and subjected to antigen retrieval in a decloaking chamber at 95° C. for 30 min in Tris-based antigen unmasking solution, pH 9 (Vector). .. After 30 min blocking, primary antibody incubation was performed at 4° C. overnight: eIF4E (SCBT), Article Title: Implications for myofibrillar protein translation due to high-intensity muscle contraction via a rapamycin-insensitive mechanism. Article Snippet: .. The membranes were washed and incubated overnight at 4°C 161 with the following primary antibodies: Phospho-p70S6K (Thr389) (#9234), p70S6K (#2708), Phospho-162 rpS6 (Ser240/244) (#2215), rpS6 (#2217), eIF4E (#9742), Article Title: Cytoskeletal protein KRT14 governs cisplatin resistance by modulating eIF4H-dependent ACOX2 translation and lipid metabolism in bladder cancer Article Snippet: Equal amounts of protein (20–40 μg) were separated on 4–15% Mini-PROTEAN TGX gels (Bio-Rad 4561086) and transferred to 0.45 μm PVDF membranes (Millipore IPVH00010). .. Membranes were blocked in TBST containing 5% non-fat dry milk or 5% BSA for 1 h at room temperature, then incubated overnight at 4 °C with primary antibodies: KRT14 (1:1000; Abcam ab181595), ACOX2 (1:1000; Proteintech 10143-1-AP), eIF4H (1:1000; CST 3469), Article Title: Methods and compositions for treating androgen receptor deficient, androgen receptor low, and castration-resistant prostate cancers Article Snippet: For IF detection, sections were incubated with secondary antibodies conjugated to Alexa-Fluor 594 (Invitrogen). .. For IF signal amplification of AR (SCBT), p-AKT S473 (CST), eIF4E (SCBT), eIF4A (CST), and Article Title: Methods and compositions for treating androgen receptor deficient, androgen receptor low, and castration-resistant prostate cancers Article Snippet: .. Permeabilized cells were washed using 1× wash buffer followed by 1 hour blocking in a humidity chamber at 37° C. Next, cells were incubated with primary antibodies: FLAG-tag:Article Title: Cytoskeletal protein KRT14 governs cisplatin resistance by modulating eIF4H-dependent ACOX2 translation and lipid metabolism in bladder cancer. Article Snippet: Equal amounts of protein (20–40 μg) were separated on 4–15% MiniPROTEAN TGX gels (Bio-Rad 4561086) and transferred to 0.45 μm PVDF membranes (Millipore IPVH00010). .. Membranes were blocked in TBST containing 5% non-fat dry milk or 5% BSA for 1 h at room temperature, then incubated overnight at 4 °C with primary antibodies: KRT14 (1:1,000; Abcam ab181595), ACOX2 (1:1,000; Proteintech 10143-1-AP), eIF4H (1:1,000; CST 3469), Article Title: Cytoskeletal protein KRT14 governs cisplatin resistance by modulating eIF4H-dependent ACOX2 translation and lipid metabolism in bladder cancer Article Snippet: Equal amounts of protein (20–40 μg) were separated on 4–15% Mini-PROTEAN TGX gels (Bio-Rad 4561086) and transferred to 0.45 μm PVDF membranes (Millipore IPVH00010). .. Membranes were blocked in TBST containing 5% non-fat dry milk or 5% BSA for 1 h at room temperature, then incubated overnight at 4 °C with primary antibodies: KRT14 (1:1000; Abcam ab181595), ACOX2 (1:1000; Proteintech 10143-1-AP), eIF4H (1:1000; CST 3469), Binding Assay:Article Title: Implications for myofibrillar protein translation due to high-intensity muscle contraction via a rapamycin-insensitive mechanism. Article Snippet: .. The membranes were washed and incubated overnight at 4°C 161 with the following primary antibodies: Phospho-p70S6K (Thr389) (#9234), p70S6K (#2708), Phospho-162 rpS6 (Ser240/244) (#2215), rpS6 (#2217), eIF4E (#9742), Amplification:Article Title: Methods and compositions for treating androgen receptor deficient, androgen receptor low, and castration-resistant prostate cancers Article Snippet: For IF detection, sections were incubated with secondary antibodies conjugated to Alexa-Fluor 594 (Invitrogen). .. For IF signal amplification of AR (SCBT), p-AKT S473 (CST), eIF4E (SCBT), eIF4A (CST), and |
![A , Western blot showing indicated proteins expression levels in YUMM1.1 cells expressing a shRNA control or targeting ASS1. HSP90 serves as a loading control. B , Western blot showing indicated proteins expression levels in YUMM1.7 cells expressing a shRNA control or targeting ASS1. HSP90 serves as a loading control. C , Western blot showing indicated proteins expression levels in YUMM2.1 cells expressing an ectopic empty vector or containing the open reading frame of ASS1. HSP90 serves as a loading control. D , Renilla over Firefly luminescent ratio quantification in YUMM1.1 cells expressing a shRNA control or targeting ASS1 (n=4; mean ± SEM). E , Renilla over Firefly luminescent ratio quantification in YUMM1.7 cells expressing a shRNA control or targeting ASS1 (n=3; mean ± SEM). F , Renilla over Firefly luminescent ratio quantification in YUMM2.1 cells expressing an ectopic empty vector or containing the open reading frame of ASS1 (n=3; mean ± SEM). G , Protein synthesis rates were determined in YUMM1.1 cells expressing a shRNA control or targeting ASS1. Cells were then pulsed for 30 min with [ 35 S]Cys/Met, and the incorporation of 35 S into proteins was quantified and normalized to the total protein amount. Cells treated 30 minutes with 100µg/mL of cycloheximide (CHX) are used as a positive control. The data are presented as the mean (n = 4). H , Protein synthesis rates were determined in YUMM1.7 cells expressing a shRNA control or targeting ASS1. Cells were then pulsed for 30 min with [ 35 S]Cys/Met, and the incorporation of 35 S into proteins was quantified and normalized to the total protein amount. Cells treated 30 minutes with 100µg/mL of cycloheximide (CHX) are used as a positive control. The data are presented as the mean (n = 3). I , Protein synthesis rates were determined in YUMM2.1 cells expressing an ectopic empty vector or containing the open reading frame of ASS1. Cells were then pulsed for 30 min with [ 35 S]Cys/Met, and the incorporation of 35 S into proteins was quantified and normalized to the total protein amount. Cells treated 30 minutes with 100µg/mL of cycloheximide (CHX) are used as a positive control. The data are presented as the mean (n = 3). J , Proximity Ligation Assay showing eiF4E/eiF4G interactions in YUMM1.1 cells expressing a shRNA control or targeting ASS1. Representative images are presented in the left panel, and quantifications are presented in the right panel. K , Proximity Ligation Assay showing eiF4E/eiF4G interactions in YUMM1.7 cells expressing a shRNA control or targeting ASS1. Representative images are presented in the left panel, and quantifications are presented in the right panel.](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_79/10__64898_slash_2026__03__17__712479/10__64898_slash_2026__03__17__712479___F3.large.jpg)
![Chronic stress pre-incubated cells fail to respond to form SGs by acute stress (A) U2OS cells were subjected to treatment with SA (10, 50, 100, and 500 μM) at each time (0, 1, 4, 12, and 24 h). Cells were examined for the presence of the core SG markers G3BP1 (green), eIF4G (red), and eIF3b (blue). Scale bars represent 10 μm. All positive cells were quantified. p values were assessed using a one-way ANOVA (vs. NT; p ∗∗∗∗ <0.0001). Results are mean ± S.E.M. ( n = 3). (B) Schematic illustration of the experimental timeline. (C–F) U2OS cells were subjected to treatment with 100 μM SA for 1 h after pre-incubation with 10 μM SA, 30 μM Mena (menadione), 2 μM Drb (doxorubicin), 1 μM Tg (thapsigargin), 25 μg/mL Tun (tunicamycin), 25 μg/mL BA (brefeldin A), 60 μM CCCP (carbonyl cyanide m -chlorophenyl hydrazone), 60 mM 2DG (2-deoxy-D-glucose), or HBSS for 24 h. Unstressed cells (NT) were used as a control. (C) Cells were examined for the presence of the core SG markers G3BP1, eIF4G, and eIF3B. (D) Representative images of U2OS cells stained with G3BP1 (green), eIF4G (red), and eIF3B (blue) after the cells had been subjected to specific stresses. Scale bars represent 20 μm. (E) Cells were examined for the presence of the core SG marker G3BP1 and poly (A) mRNAs (FISH using oligo[dT] probe). (F) Representative images of U2OS cells stained with G3BP1 (green) and oligo(dT) (red) after the cells had been subjected to specific stresses. Scale bars represent 20 μm. (C and E) p values were assessed using a one-way ANOVA (vs. 1 h SA; p ∗∗∗∗ <0.0001). Results are mean ± S.E.M. ( n = 3). All experiments were done for each of the three biological replicates (independent experiments).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8549/pmc12828549/pmc12828549__gr1.jpg)
